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GenBank CDS声明蛋白导出

按字面qualifier值筛选CDS并导出唯一声明translation，保留全部特征状态、重复qualifier与原件字节位置。

浏览器本地处理输入UTF-8核酸GenBank输出FASTA / JSON / CSV / 原件单个文件最多 10 MiB · 最多 1 个
  1. 1添加输入
  2. 2调整设置
  3. 3获取结果

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单个文件最多 10 MiB · 最多 1 个

    0 个字符 · 0 字节
    处理选项

    先填写标记为必填的选项，其余可保留默认值。

    正在准备处理工具…

    开始之前

    从完整GenBank读取现有蛋白声明。缺失、空值与重复translation都有明确状态；不从DNA重新计算蛋白。

    如何使用

    1. 选择完整GenBank文件或粘贴UTF-8文本，设置qualifier key、字面值及contains/equals规则。
    2. 查看匹配与导出数量，并核对每个无法导出的CDS状态，不假设每个匹配都有蛋白。
    3. 下载proteins.faa、完整报告与original.gb；交接声明location时一并保留原件。

    支持范围与限制

    单份UTF-8核酸GenBank，支持BOM与LF/CRLF，上限10MiB；最多10,000记录、100,000特征、20,000匹配CDS、全部特征合计8,000,000个声明translation ASCII字符、300,000物理行。原件、JSON、CSV、FASTA完整合计上限40MiB。

    物理行与声明location各限65,536个UTF-16单元；每特征最多100,000个qualifier；每个普通qualifier解码值最多1,048,576个UTF-16单元。筛选key为最多128单元的ASCII标识符，字面值最多4,096单元。所有预算共同生效，输入与输出总限约束组合容量。

    识别核酸LOCUS前缀、准确ASCII固定FEATURES列和完整ORIGIN/CONTIG加//结构。未验证完整INSDC header、DNA内容与长度、location解释、密码子、翻译表、生物有效性或外部引用。蛋白LOCUS、单独CR、非法UTF-8、不支持缩进或translation字符会拒绝。

    contains/equals按大小写敏感字面值匹配，不执行正则。缺qualifier和未匹配分别显示；仅恰好一个非空translation声明导出。没有声明为missing，一个空声明为empty，任何重复声明为ambiguous，即使其中一个为空也不擅选。

    完整JSON保留全部已解析记录与特征、按顺序重复qualifier与flags、引号形态、声明location及准确UTF-8字节跨度。FASTA header可逆包含记录/特征索引、LOCUS、全部ID与location；只移除现有translation内空白，不推断蛋白生物有效性。

    CSV包含每个CDS状态，为类似公式的文本加单引号防护；JSON保留原值。表格仅预览前200行，长单元格前2,000个UTF-16单元。超过20,000单元的复制内容是报告预览，下载仍完整；原件BOM、LF/CRLF字节精确保留。

    操作示例

    示例输入

    LOCUS       scaffold_10                 12 bp    DNA     linear   UNA 01-JAN-2000
DEFINITION  Synthetic test record; declared translations are not recomputed.
ACCESSION   SYNTH0001
VERSION     SYNTH0001.1
KEYWORDS    .
FEATURES             Location/Qualifiers
     CDS             13110..15500
                     /ID="id"
                     /NRPS_PKS="Domain: PKS_KR"
                     /translation="MKL*"
                     /NRPS_PKS="type: other"
     CDS             join(3252..3263,3321..3443,3493..4428)
                     /ID="id"
                     /NRPS_PKS="Domain: PKS_KR"
                     /translation="MKL*"
                     /ID="CDS_13111"
ORIGIN
        1 acgtacgtacgt
//
    示例参数
    {"secondary":"","params":{"qualifierKey":"NRPS_PKS","literal":"PKS_","mode":"contains"}}

    示例输出

    {"profile":"UTF8 nucleic GenBank LOCUS/FEATURES and declared CDS translations; not full INSDC/schema/sequence/location/biological validation","selection":{"qualifierKey":"NRPS_PKS","literal":"PKS_","mode":"contains","valueMatching":"literal-case-sensitive"},"source":{"encoding":"UTF-8","bytes":707,"bomRetained":false},"records":[{"index":0,"locus":"scaffold_10","declaredLengthToken":"12","accession":["SYNTH0001"],"version":"SYNTH0001.1","featureIndexes":[0,1],"source":{"utf8Start":0,"utf8Bytes":707}}],"features":[{"index":0,"record":0,"key":"CDS","qualifiers":[{"key":"ID","quoted":true,"flag":false,"source":{"utf8Start":294,"utf8Bytes":30},"value":"id"},{"key":"NRPS_PKS","quoted":true,"flag":false,"source":{"utf8Start":324,"utf8Bytes":48},"value":"Domain: PKS_KR"},{"key":"translation","quoted":true,"flag":false,"source":{"utf8Start":372,"utf8Bytes":41},"value":"MKL*"},{"key":"NRPS_PKS","quoted":true,"flag":false,"source":{"utf8Start":413,"utf8Bytes":45},"value":"type: other"}],"source":{"utf8Start":260,"utf8Bytes":198},"declaredLocation":"13110..15500"},{"index":1,"record":0,"key":"CDS","qualifiers":[{"key":"ID","quoted":true,"flag":false,"source":{"utf8Start":518,"utf8Bytes":30},"value":"id"},{"key":"NRPS_PKS","quoted":true,"flag":false,"source":{"utf8Start":548,"utf8Bytes":48},"value":"Domain: PKS_KR"},{"key":"translation","quoted":true,"flag":false,"source":{"utf8Start":596,"utf8Bytes":41},"value":"MKL*"},{"key":"ID","quoted":true,"flag":false,"source":{"utf8Start":637,"utf8Bytes":37},"value":"CDS_13111"}],"source":{"utf8Start":458,"utf8Bytes":216},"declaredLocation":"join(3252..3263,3321..3443,3493..4428)"}],"cds":[{"featureIndex":0,"recordIndex":0,"locus":"scaffold_10","ids":["id"],"declaredLocation":"13110..15500","predicateQualifierIndexes":[1,3],"matchingQualifierIndexes":[1],"translationQualifierIndexes":[2],"status":"exported"},{"featureIndex":1,"recordIndex":0,"locus":"scaffold_10","ids":["id","CDS_13111"],"declaredLocation":"join(3252..3263,3321..3443,3493..4428)","predicateQualifierIndexes":[1],"matchingQualifierIndexes":[1],"translationQualifierIndexes":[2],"status":"exported"}],"proteinManifest":[{"featureIndex":0,"header":"r=0|f=0|locus=scaffold_10|cds=%5B%22id%22%5D|location=13110..15500","aminoAcids":4},{"featureIndex":1,"header":"r=0|f=1|locus=scaffold_10|cds=%5B%22id%22%2C%22CDS_13111%22%5D|location=join(3252..3263%2C3321..3443%2C3493..4428)","aminoAcids":4}],"summary":{"records":1,"features":2,"cds":2,"matchedCDS":2,"exportedProteins":2,"exportedAminoAcids":8,"declaredTranslationCharacters":8,"physicalLines":19,"unavailableMatches":0},"unreviewed":["ORIGIN/CONTIG nucleotide content and length","location interpretation/extraction","other record headers","codon_start/transl_table consistency","biological protein validity","external references"]}

    出现问题时

    提供完整核酸记录并确认固定列和编码；在原始数据中核实筛选或重复声明，不删除证据猜选蛋白。超限时减少输入或完整交付规模。取消或失败不发布部分文件。

    常见问题

    会从DNA翻译蛋白吗？

    不会；只导出现有声明translation。DNA、密码子设置与translation一致性未审查。

    translation出现两次怎么办？

    任何重复声明都标记ambiguous并不导出，包括一个为空、另一个非空的情况。所有声明仍在完整报告中。

    会解释location吗？

    不会；join、complement、模糊位置与远端location按声明字符串及原件位置保留，不用于提取核酸。

    文档与延伸阅读

    导出声明蛋白与translation memory语言对 →保留特征与语言variant的实际含义 →

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